Coding

Part:BBa_K2996701:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-06)


RpoA fused with dcas9 through FL4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2161
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.

Source

None.

References

[1]. Gilbert L A, Larson M H, Morsut L, etc. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes[J]. Cell, 2013, 154(2): 442-451. [2]. Mathew R, Chatterji D. The evolving story of the omega subunit of bacterial RNA polymerase[J]. Trends in microbiology, 2006, 14(10): 450-5. [3]. Gourse R L, Ross W, Gaal T. UPs and downs in bacterial transcription initiation: The role of the alpha subunit of RNA polymerase in promoter recognition[J]. Molecular Microbiology, 2000, 37(4): 687-695. [4]. Murakami K S, Masuda S, Darst S A. Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution[J]. Science, 2002, 296(5571): 1280-1284.